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为探讨中药黄芩抗细菌感染及其毒血症作用的可能机制,本实验以培养内皮细胞为实验对象,以细菌内毒素为致伤剂,以中药黄芩的有效成分之一一黄芩甙为保护剂,对内毒素损伤内皮细胞以及黄芩甙可能的保护作用,从形态学和生物化学角度进行了探索.结果显示,内毒素主要引起包括线粒体肿胀、内质网扩张、细胞膜崩解在内的膜性结构的损伤,以及细胞连接破坏;黄芩甙能减轻内毒素对内皮细胞膜结构的损伤作用,尤其对细胞连接的保护作用较为明显.同时发现黄芩甙能抑制内毒素所致的细胞蛋白分泌功能增强,蛋白水解酶的释放以及氧自由基的产生等,故认为黄芩甙在一定程度上对内毒素作用的内皮细胞有保护作用.其机理与黄芩甙抑制了内毒素引起的内皮细胞的呼吸暴发和氧自由基的释放等机制有关.
In order to explore the possible mechanism of anti-bacterial and anti-toxemia effects of Astragalus membranaceus, the experiment used endothelial cells as experimental subjects, bacterial endotoxin as a wounding agent, and one of the active ingredients of Astragalus membranaceus as protective agent. The possible protective effects of endotoxin-damaged endothelial cells and jaundice were explored from a morphological and biochemical perspective. The results showed that endotoxins mainly caused membranous mitochondrial swelling, endoplasmic reticulum expansion, and cell membrane disintegration. Structural damage, and cell connection damage; Astragalus can reduce the endotoxin damage to the endothelial cell membrane structure, in particular the protection of cell connections is more obvious. At the same time found that Astragalus can inhibit the secretion of cell proteins caused by endotoxin enhanced function, The release of proteolytic enzymes and the production of oxygen free radicals suggest that Astragalus has a protective effect on endotoxin-induced endothelial cells to a certain extent. Its mechanism and Astragalus inhibit endotoxin-induced endothelial cell respiratory bursts and oxygen Free radicals and other mechanisms related to the release.