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目的探讨采用MTT法检测肿瘤化疗药对脑胶质瘤细胞的敏感性。方法分离临床实体瘤标本(原代脑胶质瘤组织)制备单细胞悬液,包括U87细胞进行体外培养,将临床上常用的8种肿瘤化疗药5-氟尿嘧啶(5-Fu)、卡铂(CBP)、顺铂(DDP)、阿霉素(DOX)、甲氨蝶呤(MTX)、依托泊苷(VP-16)、丝裂霉素(MMC)、长春新碱(VCR)配成3种不同的浓度(5-Fu的终浓度为250、500、750μg/mL,其他肿瘤化疗药终浓度50、100、150μg/mL),采用MTT法检测,计算各肿瘤化疗药物对肿瘤细胞的抑制率。结果 8种肿瘤化疗药对原代脑胶质瘤的抑制效率依次为:ADM>MMC>DDP>VCR>5-Fu>VP-16>CBP>MTX;其中ADM的敏感度最高(抑制率95.54%);其次是MMC和DDP(抑制率>60%),MTX最不敏感(11.29%)。7种化疗药物对U87细胞株的抑制率依次为:ADM>VP-16>CBP>MMC>5-Fu>DDP>MTX;ADM的抑制率最高(91.19%),最不敏感的是MTX,其次是DDP(二者<30%)。DDP对于U87细胞抑制率较低,属于耐受药,但作用于原代脑胶质瘤细胞时细胞毒作用较大,为敏感性化疗药物。结论 MTT法体外药敏试验为指导实体瘤患者的临床化疗方案提供了有效数据,可减少用药的盲目性,提高个体化治疗效果。
Objective To investigate the sensitivity of tumor chemotherapeutic drugs to glioma cells by MTT assay. METHODS: Single cell suspensions, including U87 cells, were isolated from clinical solid tumor samples (primary glioma tissue) and cultured in vitro. The 8 tumor chemotherapeutic agents commonly used in clinic, such as 5-fluorouracil (5-Fu), carboplatin (DDP), doxorubicin (DOX), methotrexate (MTX), etoposide (VP-16), mitomycin (MMC) and vincristine (VCR) (5-Fu, 250, 500, 750μg / mL, 50, 100, 150μg / mL). The MTT assay was used to calculate the inhibitory effect of each tumor chemotherapeutic agent on tumor cells rate. Results The inhibitory rates of 8 kinds of tumor chemotherapeutic agents on primary glioma were as follows: ADM> MMC> DDP> VCR> 5-Fu> VP-16> CBP> MTX; ADM had the highest sensitivity (95.54% ) Followed by MMC and DDP (inhibition rate> 60%) and MTX least sensitive (11.29%). The inhibitory rates of 7 chemotherapeutic drugs on U87 cell line were as follows: ADM> VP-16> CBP> MMC> 5-Fu> DDP> MTX; ADM had the highest inhibitory rate (91.19%); MTX was the least sensitive; Is DDP (both <30%). DDP has a low inhibitory rate on U87 cells and is a tolerant drug. However, DDP is more sensitive to chemotherapeutic drugs when acting on primary glioma cells. Conclusion MTT method in vitro susceptibility testing provides effective data for the clinical chemotherapy regimen in patients with solid tumors, which can reduce the blindness of medication and improve the individualized treatment effect.