论文部分内容阅读
DNA条形码(DNA Barcoding)可以利用一段通用基因短片段对物种(包括不同生活史阶段)进行区分。本文尝试应用该技术对深色白眉天蛾Hyles gallii(Rottemburg)、构月天蛾Parum colligata(Walker)、桃红六点天蛾Marumba gaschkewitschi(Bremer et Grey)等3种天蛾进行了鉴别。测定了3种天蛾的COⅠ基因序列,并在BOLD(Barcode of Life Data Systems)及GenBank中进行了检索。结果发现BOLD系统比GenBank更能帮助准确鉴定到物种,而在GenBank中只有1种能鉴定到种。将实验室获得序列与GenBank中下载的474条同源序列整合后,利用MEGA4.0的Kimura-2-Parameter模型进行了遗传距离分析,利用MEGA4.0及PAUP4.0进行了系统发育树的构建,结果发现待鉴定标本序列在各个系统发育树的位置基本一致,并能准确鉴定出一种天蛾。本研究表明,随着BOLD系统、GenBank等公共数据库的不断完善,DNA条形码将有助于更加有效地鉴定特定类群物种。
DNA Barcoding makes it possible to distinguish between species (including different stages of life history) by using a single generic short piece of DNA. This paper attempts to identify the three species of the moth, Hyles gallii (Rottemburg), Parum colligata (Walker), Marumba gaschkewitschi (Bremer et Gray), using the technique. The COⅠ gene sequences of three species of T. gondii were determined and searched in BOLD (Barcode of Life Data Systems) and GenBank. The results showed that the BOLD system was more able to identify the species more accurately than GenBank, whereas only one species was identified in GenBank. After integrating the obtained sequences with 474 homologous sequences downloaded from GenBank, the genetic distance was analyzed using Kimura-2-Parameter model of MEGA4.0. The phylogenetic tree was constructed by using MEGA4.0 and PAUP4.0 It was found that the sequence of the specimen to be identified was basically the same in all phylogenetic trees and could accurately identify a hawk. This study shows that with the continuous refinement of public databases such as the BOLD system and GenBank, DNA barcodes will help identify more specific taxa.