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目的：观察小牛血去蛋白提取物眼用凝胶对鼻咽癌放射治疗所致眼角膜内皮细胞损伤的影响。方法回顾研究2007年1月至2009年12月就诊于南京医科大学附属淮安市第一医院行放射治疗的放射剂量＞50 Gy 的鼻咽癌患97例，患者左右眼随机分为治疗组和对照组，治疗组应用小牛血去蛋白提取物眼用凝胶，对照组不应用，记录两组治疗前与治疗结束后1 d、1周、1个月、6个月、1年、2年的眼角膜内皮细胞密度、六边形细胞面积、内皮细胞六边形细胞比例、内皮细胞变异系数的变化。对上述4个指标采用 SPSS 12．0软件进行统计分析比较。结果治疗后1个月、3个月时，治疗组角膜内皮细胞密度较对照组明显增加，平均角膜内皮细胞面积较对照组明显减小，角膜内皮细胞变异系数较对照组明显减小，角膜内皮细胞六边形细胞较对照组明显增加，差异有统计学意义（P ＜0．05），3个月时对照组角膜内皮细胞变化程度开始减小，而治疗组相比对照组明显接近治疗前状态；其余放射治疗后不同时间同时期两组数据比较差异均无统计学意义（P ＞0．05）。结论鼻咽癌患者放射治疗前后使用小牛血去蛋白提取物眼用凝胶有利于减轻角膜内皮细胞的损伤，促进角膜内皮细胞的修复。“,”Objective To observe the effect of deproteinized calf blood extract eye gel on corneal injury reparation after nasopharyngeal carcinoma radiotherapy.Methods A retrospective study was made for 97 patients who had received radiation therapy for nasopharyngeal carcinoma between January 2007 and December 2009 in Huaian First Peoples Hos-pital,Nanjing Medical University,with the radiation dose was more than 50 Gy.The right and left eyes were divided into the treatment and control groups,the deproteinized calf blood extract eye gel was given in the treatment group.The corneal endothelial cell densities,size,hexagonal cell ratio,and coefficient of variation were ddtermined at pre-treat-ment and post-treatment 1 day,1 week,1 month,6 months,a year and 2 years in the two groups.All data were ana-lyzed by statistical analysis software SPSS 12.0.Results At 1 month and 3 months after the treatment,the corneal endothelial cell density of the treatment group was significantly increased compared with the control group,the average corneal endothelial cell area was significantly reduced,the coefficient of variation of corneal endothelial cells was signif-icantly decreased,the hexagonal cells of corneal endothelial cells were significantly increased,the difference was statis-tically significant (P <0.05).At post-treatment 3 months,changes of corneal endothelial cells in the control group began to decrease,while those in the treatment group was close to the pre-treatment state compared with the control group.The two groups had no differences in the rest time after radiotherapy.Conclusion Using deproteinized calf blood extract eye gel pre-treatment and post-treatment can help to reduce the damage to corneal endothelium and pro-mote reparation of corneal endothelial cells.