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目的：探讨肿瘤转移抑制基因ｎｍ２３的表达产物，二磷酸核苷激酶（ＮＤＰＫ）在人肝癌组织中的表达及其与预后的关系。材料和方法：材料取自河南省肿瘤医院１９８７～１９９３年肝癌切除的部分手术标本，共５４例。抗ＮＤＰＫ小鼠单克隆抗体和免疫组化试剂盒购自Ｄａｋｏ公司。研究方法按ＬＳＡＢ法免疫组织化学操作技术常规进行。结果：５４例肝癌组织中，ｎｍ２３表达阳性率为５９．３％（３２／５４），患者年龄、肿瘤的大小、肿瘤的大体分型、肿瘤分期、分级等在ｎｍ２３表达的阳性和阴性组间的分布差异无显著性性（Ｐ＞０．０５）。生存期分析表明，ｎｍ２３表达阴性组的生存期短于阳性组。结论：ｎｍ２３表达阴性与肝癌预后不良有关，但多因素分析显示，ｎｍ２３不能作为判断肝癌预后的独立指标。
Objective: To investigate the expression product of tumor metastasis suppressor gene nm23, the expression of nucleoside diphosphate kinase (NDPK) in human hepatocellular carcinoma and its relationship with prognosis. Materials and Methods: The materials were taken from some surgical specimens of hepatocellular carcinoma from 1987 to 1993 in Henan Cancer Hospital, a total of 54 cases. The anti-NDPK mouse monoclonal antibody and immunohistochemistry kit were purchased from Dako Company. The research method is routinely performed according to the LSAB immunohistochemistry technique. Results: The positive rate of nm23 expression in 54 cases of HCC was 59.3% (32/54). The age, tumor size, tumor general classification, tumor stage, and grade were among the positive and negative groups of nm23 expression. The distribution difference was not significant (P>0.05). The survival analysis showed that the survival time of the nm23 negative group was shorter than that of the positive group. Conclusion: The negative expression of nm23 is associated with poor prognosis of liver cancer. However, multivariate analysis showed that nm23 could not be used as an independent index to judge the prognosis of liver cancer.