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目的建立分子印迹流动注射化学发光法定量分析紫草素的新方法。方法在酸性条件下,紫草素对高锰酸钾-甲醛体系发光反应具有明显的增敏作用,以甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂合成紫草素的分子印迹聚合物,并将其作为分子识别物质,利用紫草素-高锰酸钾-甲醛化学发光体系,结合流动注射化学发光分析技术,建立测定紫草素的分子印迹流动注射化学发光分析方法。结果体系的化学发光强度与紫草素质量浓度呈线性关系,其标准曲线为Y=43.2 X+12.68,r=0.999 3,线性范围为1.20～80.0μg/mL,检出限为0.36μg/mL。结论利用分子印迹流动注射化学发光分析法测定紫草中的紫草素简便、快速、准确。
Objective To establish a new method for the quantitative analysis of shikonin by molecularly imprinted flow injection chemiluminescence. Methods Shikonin had obvious sensitizing effect on the luminescence of potassium permanganate-formaldehyde system under acidic conditions. Methyl acrylate was used as functional monomer with ethylene glycol dimethacrylate as the crosslinking agent The molecularly imprinted polymer was used as a molecular recognition material to establish a molecularly imprinted flow-injection chemiluminescence assay for shikonin using shikonin-potassium permanganate-formaldehyde chemiluminescence system combined with flow-injection chemiluminescence analysis Analytical method. Results The chemiluminescence intensity of the system was linear with the concentration of shikonin. The standard curve was Y = 43.2 X + 12.68, r = 0.999 3, linear range was 1.20 ~ 80.0 μg / mL and the detection limit was 0.36 μg / mL . Conclusion The molecularly imprinted flow-injection chemiluminescence assay for determination of shikonin in Lithospermum is simple, rapid and accurate.