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目的观察C5a/C5aR通路对约氏疟原虫17XL(P.y17XL)的增殖及其对BALB/c小鼠致死率的影响。结论将实验小鼠分为正常BALB/c组和C5aR-/-BALB/c组,每组5只,相同剂量的P.y17XL(2×105)分别感染正常BALB/c组小鼠和C5aR-/-BALB/c组小鼠,于感染后0、2、4和6d观察两组小鼠原虫血症和存活率的变化,并且采用ELISA检测感染P.y17XLBALB/c小鼠血清中C5a的水平。结果与正常组小鼠相比,C5aR-/-组小鼠生存期缩短,P.y17XL在C5aR-/-组小鼠的原虫血症较野生株小鼠高(P<0.05),而P.y17XL感染小鼠的血清中C5a含量低于未感染的小鼠。结论 C5a/C5aR能够抑制P.y17XL的增殖,并能延长感染小鼠的生存期。
Objective To investigate the effect of C5a / C5aR pathway on the proliferation of 17XL (P.y17XL) and its lethality in BALB / c mice. Conclusion The experimental mice were divided into normal BALB / c group and C5aR - / - BALB / c group, with 5 mice in each group. The same dose of P.y17XL (2 × 105) / -BALB / c group, the parasitemia and survival rate of the two groups were observed at 0, 2, 4 and 6 days after infection, and the level of C5a in sera of infected P.y17XLBALB / c mice was detected by ELISA . Results Compared with normal mice, C5aR - / - mice had shorter survival period and P.y17XL had higher parasitemia in C5aR - / - mice than wild mice (P <0.05). The serum levels of C5a in y17XL-infected mice were lower than in uninfected mice. Conclusion C5a / C5aR can inhibit the proliferation of P.y17XL and prolong the survival of infected mice.