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以聚己内酯和明胶为原料,通过静电纺丝制得孔隙率高、细胞分散性好、机械性能佳的三维细胞支架;扫描电镜及共聚焦显微镜表征U87细胞在该三维支架内培养14天后的生长情况,结果表明U87细胞不仅能够在支架表面黏附增殖,而且能迁移进入静电纺丝膜内部生长,形成良好的三维细胞体系.游离阿霉素(DOX)对二维表面和三维静电纺丝膜中U87细胞的细胞毒性结果表明,相对于二维细胞体系而言,三维细胞体系对药物的敏感性能降低.进一步比较了二维与三维细胞对负载于聚乙二醇-b-聚己内酯(PEG-b-PCL)胶束中DOX的响应,三维细胞体系的药物敏感性低于二维体系.
Using polycaprolactone and gelatin as raw materials, three-dimensional scaffolds with high porosity, good cell dispersion and good mechanical properties were obtained by electrospinning. U87 cells were characterized by scanning electron microscopy and confocal microscopy for 14 days in the three-dimensional scaffold The results showed that U87 cells could not only adhere and proliferate on the surface of scaffold, but also migrate into the interior of the electrospun membrane to form a good three-dimensional cell system.Doxorubicin (DOX) The cytotoxicity of U87 cells in the membrane showed that the three-dimensional cell system was less susceptible to the drug than the two-dimensional cell system.Two further studies comparing the effect of two-dimensional and three-dimensional Ester (PEG-b-PCL) micelles DOX response, three-dimensional cell system drug sensitivity than the two-dimensional system.