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目的研究国产注射用头孢拉宗钠(CFB)的体内外抗菌效果。方法体外实验采用琼脂二倍稀释法计算细菌的最低抑菌浓度(MIC),体内实验用临床分离大肠埃希菌和金黄色葡萄球菌,小鼠腹腔注射最小致死量菌液造成全身感染,静脉注射不同剂量的受试药物,观察7 d或14 d内小鼠的死亡情况,用NDST程序的Bliss法计算ED_(50)。结果 CFB对G~-菌中的大肠埃希菌、肺炎克雷伯菌抗菌作用良好,MIC50为0.25~0.5μg·mL~(-1),对G~+菌中的金黄色葡萄球菌、表皮葡萄球菌呈强抗菌作用,MIC_(50)均为0.5μg·mL~(-1),对不动杆菌和铜绿假单胞菌无抗菌活性,体内保护实验显示:国产CFB对大肠埃希菌、金黄色葡萄球菌的ED_(50)为5.71、45.00 mg·kg~(-1)。结论国产注射用CFB对体外多数G~+菌效果良好,对金黄色葡萄球菌有强抗菌作用,对G~-菌大肠埃希菌、肺炎克雷伯菌有较强抗菌作用。对腹腔感染大肠埃希菌、金黄色葡萄球菌的小鼠也有显著抗菌效果。
Objective To study the antibacterial effect of domestic injection of cefradine sodium (CFB) in vitro and in vivo. Methods In vitro experiments, the minimum bacteriostatic concentration (MIC) of bacteria was calculated by double dilution method of agar. In vivo experiments were performed with clinical isolates of Escherichia coli and Staphylococcus aureus. The mice were injected intraperitoneally with the lethal bacterial solution to cause systemic infection. Intravenous injection Different doses of the test drug were used to observe the death of mice on the 7th or 14th day. ED 50 was calculated by the Bliss method of the NDST program. Results The antibacterial activity of CFB against Escherichia coli and Klebsiella pneumoniae in G ~ - bacteria was good with a MIC50 of 0.25 ~ 0.5μg · mL ~ (-1). Staphylococcus aureus, epidermis Staphylococcus aureus was a strong antibacterial agent with MIC 50 (50) of 0.5 μg · mL -1, which showed no antibacterial activity against Acinetobacter and Pseudomonas aeruginosa. In vivo protection experiments showed that domestic CFB showed no antibacterial activity against Escherichia coli, The Staphylococcus aureus ED_ (50) was 5.71, 45.00 mg · kg -1. Conclusion Domestic injection of CFB has good effect on most of G ~ + bacteria in vitro, strong antibacterial activity against Staphylococcus aureus and strong antibacterial activity against G ~ - bacteria Escherichia coli and Klebsiella pneumoniae. On abdominal infection Escherichia coli, Staphylococcus aureus mice also have significant antibacterial effect.