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恶性疟原虫抗氯喹的机制与氯喹在耐药虫体内积聚减少有关。作者选择对氯喹敏感的恶性疟原虫HB3株在递增的氯喹量中生长。观察到随着氯喹选择压力的增加,其3号染色体体积增大,同时对氯喹敏感性降低。去除氯喹选择压力后增大的染色体恢复到原来大小,再次暴露于氯喹选择压力中,此染色体再度增大。由此可见3号染色体大小的变化与疟原虫对氯喹的敏感性有关。 经限制性酶谱分析及以3号染色体一系
The mechanism of resistance of chloroquine to Plasmodium falciparum is related to the decreased accumulation of chloroquine in resistant worms. The authors selected chloroquine-sensitive Plasmodium falciparum HB3 strains to grow in increasing amounts of chloroquine. It was observed that as the selection pressure of chloroquine increased, the size of chromosome 3 increased while the sensitivity to chloroquine decreased. Chloramphenicol removal after selection pressure increased chromosome restored to its original size, again exposed to chloroquine selective pressure, the chromosome increased again. This shows that the size of chromosome 3 changes with the chloroquine parasite susceptibility. Restriction enzyme analysis and a chromosome 3