长期以来,对感染疟原虫的按蚊均采用解剖蚊唾液腺的方法来观察蚊体内的子孢子[1],该法操作难度大、费时、准确性差,且对蚊体内未进入唾液腺的子孢子无法判断。1995年我们采用全蚊压片荧光染色的方法能简单、快速、敏感地检测蚊体内的子孢子[2],为进一步评价该方法的效果,我们做 For a long time, the use of dissecting mosquito salivary glands in mosquitoes infected with malaria parasites has been used to observe the sporozoites in mosquitoes [1]. This method is difficult, time-consuming and poorly accurate, and can not inhibit the sporozoites that do not enter the salivary glands judgment. In 1995, we adopted the method of whole mosquito tablet fluorescence staining to detect sporozoites in mosquitoes easily, rapidly and sensitively [2]. To further evaluate the effect of this method, we did