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目的探讨Notch-1在人胶质瘤细胞系U251细胞中的邻分泌机制。方法用免疫荧光组织化学方法检测单个孤一和聚集相邻的两个U251细胞的Notch-1及配体delta-like-1的表达水平。封闭U251细胞的Notch-1受体,采用SYBR荧光实时定量PCR法检测Notch-1配体delta-like-1 mRNA在不同封闭时间组的表达水平。结果聚集相邻的U251细胞Notch-1及delta-like-1的平均荧光强度高于单个孤一的细胞。封闭后delta-like-1 mRNA的表达水平明显呈时间依赖性降低。结论在U251胶质瘤细胞中,Notch-1可能通过邻分泌机制促进相邻细胞Notch-1的表达,并影响其配体delta-like-1的表达。
Objective To investigate the mechanism of Notch-1 secretion in human glioma cell line U251. Methods Immunofluorescence histochemistry was used to detect the expression of Notch-1 and its ligand delta-like-1 in two or two adjacent U251 cells. The Notch-1 receptor of U251 cells was blocked, and the expression level of Notch-1 ligand delta-like-1 mRNA at different blocking time was detected by SYBR fluorescence real-time PCR. Results The average fluorescence intensity of Notch-1 and delta-like-1 in adjacent U251 cells was higher than that of single cells. After blocking, the expression level of delta-like-1 mRNA obviously decreased in a time-dependent manner. Conclusion In U251 glioma cells, Notch-1 may promote the expression of Notch-1 in adjacent cells and affect the expression of its ligand delta-like-1 through its overexpression mechanism.