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目的研究EMP1在喉癌中的表达,探讨与临床资料的相关性。方法选取本院51例喉癌标本及其癌旁组织,应用免疫组化和Western blot技术检测EMP1蛋白表达,应用Quantitative real-time PCR技术检测mRNA表达,并分析与各临床因素之间的相关性。结果喉癌组中EMP1的蛋白及m RNA表达水平均明显低于癌旁组织。免疫组化评分经统计学分析发现EMP1的表达与病理分级有显著的相关性(P=0.02)。结论 EMP1基因在喉癌中表达明显降低,与病理分级相关。提示其在喉癌的发生发展中可能发挥抑癌基因的作用。
Objective To study the expression of EMP1 in laryngeal carcinoma and to explore the correlation with clinical data. Methods Totally 51 cases of laryngeal carcinoma and their adjacent tissues were selected for detection of EMP1 protein expression by immunohistochemistry and Western blot. Quantitative real-time PCR was used to detect the expression of EMP1 mRNA and the correlation with clinical factors . Results The expression of EMP1 protein and m RNA in laryngeal carcinoma group were significantly lower than those in paracancerous tissues. Immunohistochemical score by statistical analysis found that the expression of EMP1 and pathological grade have a significant correlation (P = 0.02). Conclusion The expression of EMP1 in laryngeal carcinoma was significantly lower than that in the pathological grade. Suggesting that it may play a role in tumor suppressor gene in the occurrence and development of laryngeal cancer.