【摘 要】
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Dear Editor,rnMost mitochondrial proteins are synthesized in the cytosol and transported into various mitochondrial subcompartments in a process that is mediated by intricate multimeric machineries.Tim23,the key component of the TIM23 complex forming a ch
【机 构】
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High Magnetic Field Laboratory,CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biolo
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Dear Editor,rnMost mitochondrial proteins are synthesized in the cytosol and transported into various mitochondrial subcompartments in a process that is mediated by intricate multimeric machineries.Tim23,the key component of the TIM23 complex forming a channel in the mitochondrial inner membrane (MIM),is believed to recognize and translocate precursor proteins into the mitochondrial matrix or to release them into the MIM.1 Previous study has demonstrated that purified Tim23 forms a protein-conducting channel that can be activated by an appropriate change in the membrane potential in the presence of a mimetic mitochondrial preprotein,2 a signaling peptide corresponding to the presequence of cytochrome c oxidase subunit V (denoted as pCoxlV,primary sequence:MLSLRQSIRFFKPATRTLCSSRYLL).Here,we present the three-dimensional (3D) solution structure of the yeast Tim23 channel in complex with pCoxlV in micelles as determined by high-resolution nuclear magnetic resonance(NMR),providing structural insight into the molecular mechanism of presequence translocation.
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