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白介素10(IL-10)抑制巨噬细胞和单核细胞的某些功能,包括肿瘤坏死因子-α(TNF-α、和白介素1的产生、前凝血质活性的诱导和氧自由基的释放。IL-10还能降低细菌脂多糖(LPS)的毒性。实验研究了IL～10在内毒素血症体外模型中的生物活性,测定了败血症病人的血浆IL-10浓度。取10例正常人的全血与1ng/ml LPS共孵育,在某些实验加入抗人体IL-10单克隆抗体(单抗)(mAb 1 F 9)拮抗剂或同型配对的对照单抗(mAb 4 B 3-12 B 3),用酶联免疫吸附法(ELISA)测IL-10和TNF-α。单用LPS刺激后分别在6小时和3小时可
Interleukin 10 (IL-10) inhibits certain functions of macrophages and monocytes including tumor necrosis factor-α (TNF-α, interleukin-1 production, pro-coagulant activity induction and oxygen free radical release. IL-10 can also reduce the toxicity of bacterial lipopolysaccharide (LPS) .In vivo, the bioactivity of IL-10 in the in vitro model of endotoxemia was measured and the concentration of plasma IL-10 in sepsis patients was measured. Whole blood was co-incubated with 1 ng / ml LPS and in some experiments anti-human IL-10 monoclonal antibody (mAb 1 F 9) antagonist or isotype matched control mAb (mAb 4 B 3-12 B 3), IL-10 and TNF-αwere detected by enzyme-linked immunosorbent assay (ELISA) .After 6 hours and 3 hours