本文叙述了构建蛋白质的定位诱变方法的最新进展。着重介绍合成中间体DNA的定位诱变;缺失、插入、区域的定位诱变;应用无意义校正tRNA的定位诱变;应用DNA片段重叠延伸的定位诱变,以及酶法体外建立基因突变库。 This article describes the latest advances in the method of site-directed mutagenesis of proteins. The mutagenesis of the DNA of the synthetic intermediate was introduced emphatically. The deletion, insertion and localization mutagenesis of the DNA were introduced. The site-directed mutagenesis of the tRNA without sense correction was applied. The site-directed mutagenesis was performed using overlapping extension of DNA fragments.