论文部分内容阅读
目的观察博莱霉素致大鼠肺纤维化中核转氯因子-κB(NF-κB)、超氧化物歧化酶(SOD)及丙二醛(MDA)的变化及丹芍化纤方对其的影响。方法选取SD大鼠30只,随机分为正常对照组(正常组)、肺纤维化模型组(模型组)和丹芍化纤方治疗组(丹芍组),每组各10只。正常组气管内滴注生理盐水,模型组和丹芍组气管内一次性灌注博来霉素5 mg/kg诱导肺纤维化,丹芍组于造模第2天开始每天给予丹芍化纤方灌胃(0.8 g/kg)治疗,正常组及模型组则给予等量生理盐水灌胃。实验第28天称大鼠体重,处死各组大鼠,称肺湿重并收集肺组织,行苏木精-伊红(HE)染色和马松(Masson)染色观察肺组织病理变化;比色法检测肺组织羟脯氨酸(HYP)、SOD及MDA变化;采用免疫组织化学的方法检测肺组织中NF-κB蛋白的表达情况。结果与正常组比较,模型组大鼠肺组织中MDA含量及NF-κB表达明显升高,而SOD酶活性则显著下降。与模型组比较,丹芍组大鼠肺组织中MDA含量及NF-κB蛋白表达明显降低,但仍较正常组高;SOD酶活性显著回升,但未恢复正常水平。结论丹芍化纤方早期应用可在一定程度上减轻博来霉素诱导的肺泡炎症反应及肺纤维化进展,对肺纤维化具有一定的治疗作用。丹芍化纤方对肺纤维化的干预机制可能与提高肺组织SOD酶活性、对抗氧化应激、抑制肺组织中NF-κB的表达、减轻肺泡炎症有关。
Objective To observe the changes of nuclear factor-κB (NF-κB), superoxide dismutase (SOD) and malondialdehyde (MDA) in bleomycin-induced pulmonary fibrosis in rats and the effect of DanShaohuixian Decoction on it . Methods Thirty SD rats were selected and randomly divided into normal control group (normal group), pulmonary fibrosis model group (model group) and Dan Shao Huanyuan Decoction group (Dan Shao group), with 10 rats in each group. Normal group, intratracheal instillation of saline, model group and Dan Shao group tracheal injection of bleomycin 5 mg / kg induced pulmonary fibrosis, Dan Shao group on the 2nd day of modeling began to give Dan Shaohua fiber irrigation Stomach (0.8 g / kg) treatment, normal group and model group were given equal volume of saline gavage. Rats were weighed and rats were sacrificed on the 28th day of the experiment. The rats were sacrificed and their lungs were weighed and wetted. The lungs were collected and stained with hematoxylin and eosin (HE) and Masson staining. Method to detect the changes of hydroxyproline (HYP), superoxide dismutase (SOD) and malondialdehyde (MDA) in lung tissue. The expression of NF-κB protein in lung tissue was detected by immunohistochemical method. Results Compared with the normal group, the content of MDA and the expression of NF-κB in the lung tissue of the model group were significantly increased, while the activity of SOD was significantly decreased. Compared with the model group, the content of MDA and the expression of NF-κB in the lung tissue of Danshen group were significantly lower than those in the normal group, but still higher than those in the normal group. The activity of SOD increased significantly, but not returned to the normal level. Conclusion Early application of Danshao Huayuan Fang can alleviate bleomycin-induced alveolar inflammation and progression of pulmonary fibrosis to a certain extent, and has a certain therapeutic effect on pulmonary fibrosis. The intervention mechanism of Danshaohuaxianfang on pulmonary fibrosis may be related to improving SOD activity in lung tissue, resisting oxidative stress, inhibiting the expression of NF-κB in lung tissue and alleviating alveolar inflammation.