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目的 检验培养基（Ｗｈｉｔｔｅｎ、ＣＺＢ、ＳＯＭ 和ＫＳＯＭ）、培养器皿（进口、国产新旧平皿）与配制培养基的水质对小鼠胚胎体外发育的影响。 方法 采用微滴培养法培养昆明种小白鼠１－细胞胚胎，以胚胎发育到囊胚的比例为判断培养效果的标准。 结果 Ｗｈｉｔｔｅｎ、ＣＺＢ、ＳＯＭ 和ＫＳＯＭ 培养基中２－细胞发育率分别为１００％ 、１００％ 、１００％ 和９８．６％ ，而囊胚发育率却分别为０％ 、７８．３％ 、４．８％ 和９．５％ ；用三蒸水、五蒸水和去离子水配制的ＣＺＢ培养基，培养囊胚发育率分别为４０．８％ 、５４．１％ 和５２．４％ ；国产和进口新平皿的囊胚发育率分别为６８．０％ 和７２．２％ ；国产和进口旧平皿的培养囊胚发育率为２７．２％ 和４６．１％ 。 结论 昆明小白鼠早期胚胎存在体外发育阻断现象；ＣＺＢ培养基培养昆明小白鼠早期胚胎效果最好；用三蒸水、五蒸水和去离子水配制的ＣＺＢ培养基培养结果无显著差别；进口和国产新平皿的培养效果相近，但再次使用时培养效果显著下降。
Objective To investigate the effects of culture media (Whitten, CZB, SOM and KSOM), culture vessels (imported and domestic new and old plates) and water quality of formulated medium on in vitro development of mouse embryos. Methods The 1-cell embryos of Kunming mice were cultured by droplet culture method, and the ratio of embryo development to blastocyst was used as the standard to judge the culture effect. Results The rates of 2-cell development in the media of Whitten, CZB, SOM and KSOM were 100%, 100%, 100% and 98.6% respectively, while the blastocyst rates were 0% and 78.3%, 4 respectively. 8% and 9.5%, respectively. The CZB culture medium with three distilled water, five distilled water and deionized water had 40.8%, 54.1% and 52.4% The blastocyst rates of imported new plates were 68.0% and 72.2% respectively. The developed blastocysts of domestic and imported old plates were 27.2% and 46.1% respectively. Conclusion The development of early embryos in Kunming mice was blocked in vitro. The early embryos of Kunming mice were best cultured in CZB medium. There was no significant difference in the culture results between CZB medium and three-distilled water, five-distilled water and deionized water. And domestic new plate culture effect is similar, but the re-use of culture effect decreased significantly.