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对 3,5 二硝基苯甲酰基衍生化的 β 环糊精键合固定相进行研究 ,采用均匀设计法对衍生化反应的工艺条件进行优化 .用红外光谱、元素分析及热重分析方法对键合相进行表征 ,结果表明此键合相性能稳定 ,适用于正相及反相液相色谱 .用 3,5 二硝基苯甲酰化的 β 环糊精键合相做薄层色谱的手性固定相 ,结合氯仿 丙酮 三乙胺和乙腈 φ =1%乙酸三乙胺 乙酸展开剂系统 ,7种手性药物得到完全分离 ,对映体间的相对比移值 α 为 1 19～ 2 0 3.
The 3,5-dinitrobenzoyl-derivatized β-cyclodextrin bonded phase was studied, and the uniform design method was used to optimize the derivatization reaction conditions.Using infrared spectroscopy, elemental analysis and thermogravimetric analysis The results showed that the bonded phase was stable and suitable for normal phase and reversed-phase liquid chromatography. The 3,5-dinitrobenzoylated β-cyclodextrin bonded phase was characterized by thin layer chromatography Chiral stationary phase, combined with chloroform acetone triethylamine and acetonitrile φ = 1% triethylamine acetate acetic acid developing agent system, seven kinds of chiral drugs were completely separated, the relative enantiomer relative displacement α was 19 ~ 2 0 3.