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目的研究肥胖患者的肠道菌群在无菌小鼠体内的定植规律。方法选取20只无菌KM小鼠,接种肥胖患者的粪便,构建菌群人源化(HFA)动物模型,利用变性梯度凝胶电泳技术(DGGE)评价患者肠道菌群在无菌小鼠体内的定植规律。结果 HFA小鼠菌群平均丰富度(richness,S)为12.04±3.68,肥胖患者的条带S为24,为HFA小鼠S的2倍;肥胖患者Shannon指数(H’)为3.02,HFA小鼠平均H’为2.46±0.33;HFA小鼠与人肠道菌群的总相似度为26%;大部分雌性HFA小鼠与雄性HFA小鼠在聚类分析图上分离,且雄性HFA小鼠与患者更为相似。结论HFA小鼠体内能部分模拟肥胖患者的微生物区系,且与患者性别相同的小鼠模拟得更好。本实验建立的HFA模型为肥胖与肠道菌群关系的进一步研究提供新的选择。
Objective To study the colonization of intestinal flora in obese patients in sterile mice. Methods 20 sterile KM mice were selected and inoculated with stools from obese patients to construct a model animal population of humanized HFA. Denaturing gradient gel electrophoresis (DGGE) was used to evaluate the intestinal microflora of the mice in sterile mice Colonization rules. Results The average richness (S) of HFA mice was 12.04 ± 3.68, while that of obese patients was 24, which was twice of that of HFA mice. The Shannon index (H ’) of obese patients was 3.02 and HFA was small The average H ’of the mice was 2.46 ± 0.33; the total similarity between HFA mice and human intestinal flora was 26%; most of the female HFA mice and the male HFA mice were separated on the cluster analysis map and the male HFA mice Much more similar to the patient. Conclusion HFA mice can partially mimic the microflora of obese patients and simulate better in mice with the same gender. The HFA model established in this experiment provides a new choice for further research on the relationship between obesity and intestinal flora.