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化妆品细菌总数的测定是验证化妆品卫生质量的重要标志。但在进行某些化妆品的细菌总数检测时,常遇到菌落与化妆品中小颗粒难以区分的问题,以致影响实验结果的观察。为此,我们根据测定细菌脱氢酶的原理,用氯化三苯四氮唑(TTC)加以指示,使培养后的菌落呈现红色进行菌落计数,从而提高了测定化妆品细菌总数的准确性和可靠性。1 材料和方法 1.1 材料 氯化三苯四氮唑(简称TTC)由上海试剂三厂生产。卵磷脂吐温琼脂培养基(简称SCDLP琼脂培养基)由北京海淀区微生物
The determination of the total number of cosmetic bacteria is an important symbol to verify the hygienic quality of cosmetics. However, the total number of bacteria in the detection of certain cosmetics, often encountered in colonies and cosmetics difficult to distinguish the small particles, so as to affect the experimental results observed. To this end, according to the principle of measuring bacterial dehydrogenase, we use trichloro-tetrazolium chloride (TTC) to instruct the cultured colonies to be red for colony counting, thereby improving the accuracy and reliability of determining the total number of cosmetic bacteria Sex. 1 Materials and methods 1.1 Materials Trichloride chloride (referred to as TTC) by the Shanghai Reagent Factory production. Lecithin Tween agar medium (referred to as SCDLP agar medium) from Haidian District, Beijing microbes