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目的观察大鼠骨癌痛时脊髓小胶质细胞以及IL-1β的变化。方法 96只雌性SD大鼠随机分为假手术组(Sham组)和CIBP组,每组48只。CIBP组大鼠胫骨内注射Walker256乳腺癌细胞构建骨癌痛模型。于注射瘤细胞前和后6、14、21d取出L_4~L_6节段脊髓,检测IL-1β的变化趋势,利用免疫组化方法进一步明确小胶质细胞标记物(OX-42)在脊髓背角的分布及表达情况。结果与Sham组比较,接种瘤细胞后6d,CIBP组大鼠术侧脊髓背角OX42积分光密度值明显增加(P<0.01)。可见肿瘤细胞的植入能诱导大鼠同侧脊髓背角OX-42的表达显著增加,并使小胶质细胞的胞体变得肥大。CIBP组IL-1β于接种肿瘤细胞后6d开始表达增加,在整个观察期内持续高表达,明显高于Sham组(P<0.01)。结论大鼠胫骨接种肿瘤细胞后,脊髓背角内小胶质细胞被广泛激活,IL-1β释放增加。
Objective To observe the changes of spinal cord microglial cells and IL-1β in rats with bone cancer pain. Methods 96 female SD rats were randomly divided into sham operation group (Sham group) and CIBP group (48 rats in each group). Construction of bone cancer pain model by injecting Walker256 breast cancer cells into the tibia of CIBP rats. The changes of IL-1β in the spinal cord of L_4 ~ L_6 were detected before and 6, 14 and 21 days after injection of tumor cells. Immunohistochemistry was used to further confirm the expression of microglial marker (OX-42) The distribution and expression of the situation. Results Compared with Sham group, on day 6 after inoculation of tumor cells, the OX42 integral optical density value of spinal dorsal horn increased significantly in CIBP group (P <0.01). It can be seen that the implantation of tumor cells can induce a significant increase of OX-42 expression in the dorsal horn of the ipsilateral spinal cord and enlarge the cell bodies of microglial cells. The expression of IL-1β in CIBP group began to increase 6 days after inoculation of tumor cells, and continued to be high throughout the observation period, which was significantly higher than that in Sham group (P <0.01). Conclusion After inoculation of tumor cells in rat tibia, the microglia in the dorsal horn of the spinal cord are widely activated and the release of IL-1β is increased.