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目的:建立了测定大鼠血浆和房水中芒果苷含量的液相色谱检测方法。方法:以对硝基苯酚作内标,甲醇-2%冰醋酸(40∶60)为流动相,流速为1.0 mL.min-1。在Cosmosil ODS C18色谱柱(4.6 mm×250 mm,5μm)上进行分离。结果:方法的线性范围分别为血浆0.50～250.00 mg.L-1,房水0.10～10.00 mg.L-1,线性回归系数均在0.995 4以上,日内和日间精密度RSD均小于12%,最低检量限0.05 mg.L-1。结论:本方法具有简便、准确等优点,可满足芒果苷在大鼠血浆和房水中药动学研究的需求。
OBJECTIVE: To establish a liquid chromatography method for the determination of mangiferin in rat plasma and aqueous humor. Methods: P-nitrophenol was used as internal standard. Methanol-2% glacial acetic acid (40:60) was used as the mobile phase at a flow rate of 1.0 mL.min-1. Separation was performed on a Cosmosil ODS C18 column (4.6 mm × 250 mm, 5 μm). Results: The linear range of the method was 0.50 ~ 250.00 mg.L-1 in plasma and 0.10 ~ 10.00 mg.L-1 in aqueous humor respectively. The linear regression coefficients were all above 0.995 4. The intra-day and inter-day RSD were less than 12% The minimum limit of detection 0.05 mg.L-1. Conclusion: The method is simple, accurate and can meet the needs of pharmacokinetics of mangiferin in rat plasma and aqueous humor.