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目的了解昆明地区肠黏附性大肠埃希菌(Enteroaggregative Escherichia coli,EAEC)的流行特征,为防治EAEC感染提供科学参考。方法收集云南省昆明市4家哨点医院的腹泻患者粪便标本1 121份,非腹泻者的粪便标本319份,进行细菌培养(麦康凯培养基),无菌挑选单个菌落(8-12个),采用热提取法提取细菌DNA,采用荧光定量PCR检测EAEC,同时使用结构化问卷收集研究对象的基本信息。结果 EAEC在腹泻患者和非腹泻者中的检出率分别为5.4%和1.3%,差异有统计学意义(χ2=10.104,P<0.05)。男、女腹泻患者EAEC检出率分别为5.2%和5.7%,差异无统计学意义(χ~2=0.140,P>0.05);不同年龄组腹泻患者中EAEC检出率差异无统计学意义(χ~2=10.865,P>0.05);腹泻患者EAEC检出率有明显的季节分布特征(χ~2=12.819,P<0.05),夏季检出率最高(10.5%,21/200);冬季最低(2.0%,8/394)。EAEC阳性腹泻患者水样便占26.2%(16/61),黏液样便占70.5%(43/61)。EAEC腹泻患者易出现呕吐症状(χ~2=12.819,P<0.05)。结论 EAEC是腹泻的重要细菌性病原体,开展和加强EAEC的监测,可为EAEC相关腹泻的预防和控制提供科学依据。
Objective To understand the epidemiological characteristics of Enteroaggregative Escherichia coli (EAEC) in Kunming and provide a scientific reference for the prevention and treatment of EAEC infection. Methods A total of 1 121 stool samples from diarrhea patients and 319 stool samples from non-diarrhea patients were collected from 4 sentinel hospitals in Kunming, Yunnan Province. Bacterial culture (MacConkey medium) and single colonies (8-12) , Bacterial DNA was extracted by heat extraction, EAEC was detected by real-time quantitative PCR, and the basic information of subjects was collected by structured questionnaire. Results The detection rates of EAEC in diarrhea patients and non-diarrhea patients were 5.4% and 1.3%, respectively, with statistical significance (χ2 = 10.104, P <0.05). The detection rates of EAEC in male and female patients with diarrhea were 5.2% and 5.7% respectively, with no significant difference (χ ~ 2 = 0.140, P> 0.05). There was no significant difference in the detection rate of EAEC between diarrhea patients of different age groups (χ ~ 2 = 10.865, P> 0.05). The detection rate of EAEC in diarrhea patients was obviously seasonal distribution (χ ~ 2 = 12.819, P <0.05) and the highest in summer (10.5%, 21/200) Lowest (2.0%, 8/394). In EAEC positive diarrhea patients, water samples accounted for 26.2% (16/61) and mucoid samples accounted for 70.5% (43/61). Patients with EAEC diarrhea were prone to vomiting (χ ~ 2 = 12.819, P <0.05). Conclusion EAEC is an important bacterial pathogen of diarrhea. To carry out and strengthen the monitoring of EAEC may provide a scientific basis for the prevention and control of EAEC-related diarrhea.