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目的 研究日本血吸虫病经胎盘传播宿主的血清免疫反应。 方法 5只怀孕晚期母兔 ,感染日本血吸虫尾蚴5 0 0条 /只 ,仔兔出生后 4 3d起 ,每隔 2周采集血清 1次 ,至仔兔发育到成兔 (出生后 113d) ,然后以 EL ISA检测仔兔日本血吸虫特异性 Ig G、Ig M抗体。 结果 6 0 % (12 / 2 0 )仔兔经胎盘感染日本血吸虫 ,其中双性感染 5只 ,肝脏均见虫卵结节 ;单性感染 7只。仔兔血清 Ig M抗体均为阴性 (A值为 0 .0 2～ 0 .2 8,阳性对照为 0 .4 0～ 0 .5 7) ;仔兔血清 Ig G抗体 ,肝脏有虫卵结节的 5只双性感染仔兔中 ,1只出生后 5 7d起、2只出生后 71d起、1只出生后 85 d起呈阳性。其余仔兔 Ig G抗体均为阴性。 结论 经胎盘感染日本血吸虫家兔 ,其所产仔兔在短期内可产生免疫耐受性
Objective To study the serum immune response of schistosomiasis japonica placenta transmitting host. Methods Five pregnant females were infected with 500 cercariae of Schistosoma japonicum at 4 days after birth. Serum was harvested once every 2 weeks and then developed into rabbits (113 days after birth). Then, Detection of Schistosoma japonicum Specific Ig G, Ig M Antibody by EL ISA. Results Sixty percent (12/200) of the rabbits were infected with the placenta through Schistosoma japonicum. Five of them were bisexual and all had hepatic tuberculosis and seven of them were unilateral infection. Rabbit serum Ig M antibodies were negative (A value of 0. 02 ~ 0.28, the positive control was 0. 0 ~ 0. 5 7); rabbit serum Ig G antibody, the liver has egg nodules Of the 5 bisexual infected rabbits, one after birth from 57d, two from 71d after birth, one after 85 days since birth was positive. The remaining rabbit IgG antibodies were negative. Conclusion Infection of rabbit with Schistosoma japonicum through placenta can produce immunological tolerance in short term