论文部分内容阅读
目的观察新型聚内酯微泡的体外物理化学特性及声学特性,以评价其作为超声造影剂的可行性。方法采用水/油/水乳液-溶剂蒸发法制备聚内酯中空微泡,部分在制备过程中加入荧光剂。光学显微镜、扫描电镜及激光共聚焦显微镜观察微泡大小形态及内部特征;Coulter计数仪测定粒径分布和微泡浓度;体外声学装置测试微泡的体外背向散射和声衰减特性。结果该新型聚内酯超声造影剂为含空气的冻干粉剂,扫描电镜和激光共聚焦显微镜证明该聚内酯微球是中空微泡,平均粒径1.5μm,98%的微泡粒径<8μm,0.1 g多聚体微泡干粉溶于5 ml蒸馏水中的浓度为0.1×109~1×109/ml。体外声学测试微泡具有较强的散射性能。结论该聚内酯微泡符合作为超声造影剂的条件,是一种新型的超声造影剂。
Objective To observe the physico-chemical properties and acoustical properties of novel polylactone microbubbles in vitro to evaluate their feasibility as ultrasound contrast agents. Methods Poly (lactone) hollow microbubbles were prepared by water / oil / water emulsion-solvent evaporation method, and part of fluorescein was added during the preparation. Light microscopy, scanning electron microscopy and laser confocal microscopy were used to observe the size and shape of the microbubbles. Coulter counter was used to determine the particle size distribution and microbubble concentration. The in vitro acoustic device was used to measure the in vitro scattering and attenuation characteristics of microbubbles. Results The new type of polylactone ultrasound contrast agent was air-containing lyophilized powder. Scanning electron microscopy and laser scanning confocal microscopy demonstrated that the polylactone microspheres were hollow microbubbles with average diameter of 1.5 μm and particle size of 98% 8μm, 0.1 g multimeric microbubbles powder dissolved in 5 ml of distilled water at a concentration of 0.1 × 109 ~ 1 × 109 / ml. In vitro acoustic test microbubbles have strong scattering properties. Conclusion The polylactone microbubbles meet the requirements of ultrasound contrast agent is a new type of ultrasound contrast agent.