论文部分内容阅读
作者结扎家兔左冠状动脉前降支,对急性心肌缺血时蛋白质的降解和氨基酸代谢进行了研究。用离子交换色谱法测定缺血心肌组织游离氨基酸(酸性、中性、碱性)以及氨含量,发现缺血时大多数游离氨基酸含量上升,丙氨酸、缬氨酸、异亮氨酸和组氨酸含量在缺血15min即有明显增加,但苏氨酸、谷氨酸和游离氨基酸总量却随缺血时间的延长而逐渐下降。丝氨酸含量在缺血早期上升,缺血60～480min,含量无明显变化。缺血120min以后,甘氨酸和精氨酸含量逐渐下降。组织氨含量在缺血早期上升,晚期下降。游离氨基酸总量、苏氨酸、甘氨酸、精氨酸以及组织氨含量的降低是缺血心肌氨基酸代谢的特征性变化。作者认为:测定缺血心肌游离氨基酸及组织氨含量有助于急性心肌缺血的早期诊断。
The authors ligated the left anterior descending coronary artery in rabbits and studied protein degradation and amino acid metabolism during acute myocardial ischemia. Determination of free amino acids (acidic, neutral, alkaline) and ammonia content in ischemic myocardium by ion-exchange chromatography showed that most of the free amino acids were increased during ischemia, with alanine, valine, isoleucine and group Serum content of 15min significantly increased after ischemia, but the total amount of threonine, glutamic acid and free amino acids gradually decreased with prolonged ischemia. Serine content increased early in ischemia, ischemia 60 ~ 480min, no significant changes in content. After 120 min of ischemia, the content of glycine and arginine gradually decreased. Tissue ammonia content increased early in ischemia, decreased late. The reduction of total free amino acids, threonine, glycine, arginine and tissue ammonia content is characteristic of amino acid metabolism in ischemic myocardium. The authors believe: Determination of free amino acids in ischemic myocardium and tissue ammonia content contribute to the early diagnosis of acute myocardial ischemia.