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Sialosyl-T是一种粘蛋白糖类相关抗原、可作为肿瘤标记物在许多癌肿中表达.胃粘膜肠腺化生以产生粘蛋白为特征,是胃癌发生机制的重要病变.作者对1979～1985年手术治疗127例胃癌进行临床随访和免疫组织化学研究,探讨胃癌组织中Sialosyl-T抗原表达及其生物学行为.用10%福尔马林固定组织标本并石蜡包埋,作4μm厚连续切片,苏本紫和伊红染色.以单克隆TKH_2测定Sialosyl-T抗原,其组织定位采用免疫组织化学ABC法.在室温下将一抗加入以1:10稀释0.05mol磷酸缓冲液中1小时,切片用生物素标记的山羊抗体IGg孵育.正常鼠血清代替一抗孵育作阴性对照切片.临床研究包括组织分级,淋巴结状况和浸润深度,组织分化程度为良好和差两种类型.不考虑淋巴结清扫部位和数量;但要评估胃癌切片的侵润深度,分为粘膜下层和深入肌层两组.采用美国Epies751型流式细胞仪分析细胞核DNA含量.福尔马林固定石蜡包埋标本切成5μm和40μm切片,前者行伊红染色用以确定病变,后者测定DNA含量.根据Schutt’s方法制备单细胞混悬液,大多数标本均含有纤维细胞、淋巴细胞和上皮细胞作为内部标准.DNA图像显示单峰为二倍体,双峰以上为异倍体.变异系数<8%为理想病例.Wilcoxon检验分析Kaplan-Meier生存曲线.X~2检验分析各种预后因素和Sialosyl-T抗原表达
Sialosyl-T is a mucin-carbohydrate-related antigen that can be expressed as a tumor marker in many cancers. Gastric mucosal intestinal metaplasia is characteristic of mucin production and is an important pathological mechanism of gastric cancer. Surgical treatment of 127 cases of gastric cancer was performed in 1985 for clinical follow-up and immunohistochemical studies to investigate the expression and biological behavior of Sialosyl-T antigen in gastric cancer tissues. Tissue specimens were fixed in 10% formalin and paraffin-embedded for continuous 4μm thickness. Sections were stained with SuBen purple and eosin. Sialosyl-T antigen was determined by monoclonal TKH_2. Histochemical localization was performed by immunohistochemical ABC method. The primary antibody was added to 1:10 dilution of 0.05mol phosphate buffer for 1 hour at room temperature. Sections were incubated with biotin-labeled goat antibody IGg. Normal mouse serum was used as a negative control instead of primary antibody incubation. Clinical studies included tissue grading, lymph node status and depth of invasion, and the degree of tissue differentiation was good and poor. The lymph nodes were not considered. Cleaning site and quantity; but to assess the invasion depth of gastric cancer slices, divided into submucosa and deep muscle layers. Using the US Epis751 flow cytometry analysis of nuclear DNA content. Marlin-fixed paraffin-embedded specimens were cut into 5 μm and 40 μm sections. The former was stained with eosin to determine the lesions, and the latter was used to determine DNA content. Single cell suspensions were prepared according to Schutt’s method. Most specimens contained fibrocytes and lymphocytes. And epithelial cells as an internal standard. DNA images show that the unimodality is diploid and the bimodal is heteroploid. The coefficient of variation is less than 8%. It is an ideal case. Wilcoxon test analysis Kaplan-Meier survival curve. X~2 test analysis of various Prognostic factors and Sialosyl-T antigen expression