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結核菌分离培养时,除了应用富于营养的培基外,还必須选择适宜的前处理方法,才能提高阳性率,达到細菌学診断目的。前处理方法原則上要求:(1)不影响結核菌的生长;(2)使标本中杂菌死灭;(3)容易液化;(4)操作簡便。在前处理消化剂中一般采用酸类及碱类。近年来亦有主张应用其他化学物质者。虽然文献上已有許多报告,但意見尚不一致。我們对結核菌分离培养时如何选择前处理消化剂及离心沉淀与否等問題,进行了試驗,报告如下。
Mycobacterium tuberculosis isolation and culture, in addition to the use of nutrient-rich culture base, but also must choose the appropriate pre-treatment methods, in order to improve the positive rate, to achieve the purpose of bacteriological diagnosis. Pre-treatment methods in principle require: (1) does not affect the growth of Mycobacterium tuberculosis; (2) the bacteria in the specimen died; (3) easy to liquefy; (4) easy to operate. In the pre-treatment of digestive agents commonly used acids and bases. In recent years, there are also those who advocate the use of other chemical substances. Although there are many reports in the literature, the opinions are still not consistent. We TB on the isolation and culture of how to choose pre-treatment of digestive and centrifugal precipitation or not, were tested, the report is as follows.