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应用放射免疫分析法测定大鼠纹状体、下丘脑、丘脑、桥延脑内甲啡肽和亮啡肽样免疫活性物质(Ir-MEK,Ir-LEK)的含量。脑室注射氨基肽酶抑制剂 bestatin(B)或“脑啡肽酶”抑制剂 thiorphan(T)各50μg 并不影响脑内 Ir 脑啡肽含量,合并应用 B+T 各50μg 仅引起下丘脑 Ir-MEK 含量轻度上升。这说明安静状态下中枢脑啡肽的更新率不高。给大鼠电针30min 使纹状体和下丘脑 Ir-MEK 和 Ir-LEK 含量升高约40%(33—52%)(P<0.01)。在脑室注射 B+T 各100μg 以及腹腔注射非特异性肽酶抑制剂 D-苯丙氨酸250mg/Kg的基础上电针,则使 Ir-MEK 和 Ir-LEK 含量升高约120%(94—147%)(P<0.01)。以上结果说明30min 电针刺激既促进脑啡肽的合成,也促进其释放。由于前者超过后者,因此静态含量升高。在中枢脑啡肽含量升高的同时,电针镇痛效果加强。说明由于肽酶抑制剂的保护作用而积聚于脑内的脑啡肽是具有功能意义的。
Radioimmunoassay was used to determine the contents of encephalitin and leu-enkephalin-like immunoreactive substance (Ir-MEK, Ir-LEK) in striatum, hypothalamus, thalamus and bridging brain in rats. Intravenous injection of aminopeptidase inhibitor bestatin (B) or “enkephalinase” inhibitor thiorphan (T) of 50μg does not affect brain enkelin content, combined with B + T of 50μg only cause hypothalamic Ir- MEK slightly increased. This shows that the resting state of central enkephalin is not high update rate. Electro-acupuncture at 30 min in rats increased the levels of Ir-MEK and Ir-LEK in the striatum and hypothalamus by about 40% (33-52%) (P <0.01). Electro-acupuncture on the basis of intracerebroventricular injection of 100 μg B + T and intraperitoneal injection of non-specific peptidase inhibitor D-phenylalanine 250 mg / Kg increased the levels of Ir-MEK and Ir-LEK by about 120% (94- 147%) (P <0.01). The above results show that 30min electroacupuncture stimulation not only promote enkephalin synthesis, but also promote its release. As the former exceeds the latter, the static content is increased. In the central enkephalin content increased, acupuncture analgesic effect. Enkephalin, which accumulates in the brain due to the protective effect of peptidase inhibitors, is of functional significance.