头孢三嗪(Ceftriaxone)是具有广谱抗菌作用的第三代头孢菌素。测定方法有微生物法、分光光度法、极谱法和HPLC法等,本文介绍一种离子对HPLC法,离子对使强极性的头孢三嗪的保留时间在合适范围内。色谱条件5μm Lichrospher RP 18反相不锈钢柱(250×4.6 mm i.d.),流动相为乙腈-pH 7磷酸缓冲液-重蒸馏水(54∶5∶41),加入4 g十六烷基三甲基溴化铵作为相 Ceftriaxone is the third generation cephalosporin with broad spectrum antibacterial activity. Determination of microbial methods, spectrophotometry, polarography and HPLC method, this article describes an ion-pair HPLC method, the ion pair to make the strong-polar cefotaxime retention time within the appropriate range. The chromatographic conditions were 5 μL Lichrospher RP 18 reversed-phase stainless steel column (250 × 4.6 mm id) with mobile phase of acetonitrile-pH 7 phosphate buffer-double distilled water (54: 5:41), 4 g of hexadecyl trimethyl bromide Ammonium as a phase