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目的 探讨尖锐湿疣 ( CA)细胞凋亡与调节和增殖的关系及其意义。方法 采用 TUNEL 法对2 8例 CA患者的角质形成细胞凋亡进行了原位检测 ,并用免疫组化法观察了 Fas、bcl- 2及 Ki- 6 7的阳性表达。结果 本组病例中的 2 4例 ( 85 .7% )有凋亡细胞检出 ,2 8例 ( 10 0 % )有 Fas和 Ki- 6 7阳性表达 ,但 bcl- 2仅 8例 ( 2 8.6 % )有中、低度的表达。其凋亡指数 ( AI)、Fas、bcl- 2及 Ki- 6 7阳性表达与病情严重度之间无明显关系 ( P>0 .0 5 )。AI与Fas表达呈显著正相关 ( r=0 .86 6 ,P=0 .0 0 0 ) ,与 bcl- 2及 Ki- 6 7呈显著负相关 ( r=- 0 .416 ,P=0 .0 18;r=- 0 .475 ,P=0 .0 0 6 )。结论 CA中角朊细胞的凋亡可能在限制病毒的繁殖上起重要作用 ,Fas对凋亡具上调作用 ,bcl- 2有抑制作用 ,Ki- 6 7过度表达可能与细胞周期加速有关
Objective To investigate the relationship between apoptosis and regulation and proliferation of condyloma acuminatum (CA) and its significance. Methods Apoptosis of keratinocytes in 28 CA patients was detected by TUNEL method. The expressions of Fas, bcl-2 and Ki- 6 7 were observed by immunohistochemistry. Results Apoptotic cells were detected in 24 cases (85.7%) in this group of patients, 28 cases (100%) had positive expression of Fas and Ki-67 but only 8 cases of bcl-2 %) Have medium and low expression. There was no significant correlation between AI, Fas, bcl-2 and Ki- 67 expression and severity of disease (P> 0.05). There was a significant positive correlation between AI and Fas expression (r = 0.86 6, P = 0.0000), but negatively correlated with bcl-2 and Ki-67 (r = -0.416, P = 0 18; r = - 0 .475, P = .0 0 6). Conclusion Apoptosis of keratinocytes in CA may play an important role in limiting the propagation of the virus. Fas may up-regulate the apoptosis and inhibit bcl-2. The overexpression of K-6 7 may be related to the accelerated cell cycle