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cDNA文库的构建和筛选是基因克隆的重要方法之一,它是目前发现新基因和研究基因功能的基本工具。牧草是陆地植物生态系统的重要组成部分,在畜牧业发展中起重要作用。cDNA文库构建研究已经应用于紫花苜蓿等多种牧草的遗传育种学研究,获得了很多与目标性状紧密相关的功能基因。文章就近年来cDNA文库的构建及其在牧草育种研究中的应用作一综述。
The construction and screening of cDNA library is one of the important methods for gene cloning. It is the basic tool for discovering new genes and studying gene function. Pasture is an important part of the terrestrial plant ecosystem and plays an important role in animal husbandry development. The research of cDNA library construction has been applied to the genetic breeding of alfalfa and other grasses and many functional genes have been obtained which are closely related to the target traits. In this paper, the construction of cDNA library in recent years and its application in forage breeding research are reviewed.