目的:研究人类系统性红斑狼疮(systemiclupuserythematosus,SLE)血清对正常人单核细胞(monocyte,Mo)分化形成的树突状细胞(dendriticcell,DC)的表型成熟的影响。方法:分离正常人外周血单个核细胞(PBMC),加入内皮细胞穿越模型中,分别以正常人血清和SLE血清加入培养体系中,36h后收集2次穿越内皮单层得到的,经形态学鉴定的DC细胞,流式细胞术测DC的CD80、CD86、HLA鄄DR的表达情况。结果:SLE血清诱导的DC其CD80的表达水平明显低于正常人血清刺激组,而二者均高表达CD86、HLA鄄DR。结论:DC的表型成熟和血清环境有一定关系。 OBJECTIVE: To study the effect of serum of systemic lupus erythematosus (SLE) on the phenotypic maturation of dendritic cells (DCs) derived from normal human monocytes (Mo). Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from normal human peripheral blood mononuclear cells (PBMCs) and added into endothelial cells. The normal human serum and SLE serum were added to the culture system. After 36h, Of DC cells, flow cytometry DC CD80, CD86, HLA-DR expression. Results: The expression of CD80 in SLE serum-induced DCs was significantly lower than that in normal human serum-stimulated groups, both of which expressed CD86 and HLA-DR highly. Conclusion: The phenotypic maturation of DC is related to the serum environment.