目前鼠疫间接血凝试验仍是鼠疫检测中的重要手段,其中关键是F:致敏血球质量。过去以小量实验室制备的程序用于大量生产,质量不稳定,甚至失败。因此笔者探索了大量制备的程序。 予试验合格的公绵羊全采血,与等量阿氏液(Alsever’s)混合,4℃保存,2日后,离心去阿氏液,用PH7.0生理盐水洗5次,血球容积与洗液比大于1:10,每次3500～4000r/min 5min(以下洗涤步骤相同),再用生理盐水制成25％血球液,取其1.5份与1份2.5％戊二醛液混合(滴加要慢),后于20℃振荡温育3h,离心去上清液,用生理盐水洗3次,并制 At present, indirect hemagglutination test of plague is still an important method in plague detection. The key point is F: sensitized blood cell mass. In the past, a small laboratory-based program was used for mass production, the quality was unstable, and even failed. So I explored a large number of preparation procedures. All the ramses that had passed the test were collected blood and mixed with the same amount of Alsever’s and stored at 4 ° C. After 2 days, they were centrifuged to remove the Aleurites and washed five times with PH 7.0 normal saline. The ratio of hematocrit to lotion was greater than 1:10, each 3500 ~ 4000r / min 5min (the same washing steps the same), and then made of 25% normal saline blood cells, take 1.5 parts and 1 2.5% glutaraldehyde mixed solution (dropwise to slow) , After incubation at 20 ℃ oscillation 3h, the supernatant was centrifuged, washed three times with saline and system