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目的研究我国临床分离阿米卡星耐药大肠埃希菌中16S rRNA甲基化酶基因及其水平转移情况。方法用PCR法检测16S rRNA甲基化酶基因(arm A、rmt A、rmt B、rmt C、rmt D、rmt E和npm A),用液体培养基传递法研究其水平转移情况。结果 183株对阿米卡星耐药大肠埃希菌中,arm A基因阳性率为12.57%,rmt B基因阳性率为53.00%,rmt B基因阳性率在2007至2008年、2009至2010年、2011至2012年这3个年度之间比较差异有统计学意义。arm A和rmt B基因均可在大肠埃希菌间水平转移。结论 16S rRNA甲基化酶基因以arm A和rmt B为主要流行型类别,后者的分离率较高且其阳性率呈显著的上升趋势。
Objective To study the 16S rRNA methylase gene and its level metastasis in clinical isolates of Amikacin-resistant Escherichia coli in China. Methods The 16S rRNA methylase gene (arm A, rmt A, rmt B, rmt C, rmt D, rmt E and npm A) was detected by PCR. The level of 16S rRNA methylation was detected by liquid culture medium transfer assay. Results The positive rate of arm A gene in the 183 strains of Amyco-resistant Escherichia coli was 12.57% and the positive rate of rmt B gene was 53.00%. The positive rate of rmt B gene in 2007-2008, 2009-2010, The difference between the three years from 2011 to 2012 was statistically significant. Both arm A and rmt B genes can be transferred horizontally between Escherichia coli. Conclusion 16S rRNA methylase genes are arm A and rmt B, and the latter have a high isolation rate and a significant upward trend.