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目的研究DNA结合分化抑制蛋白1(Id-1)在结直肠癌细胞增殖、分化过程中的重要作用。方法选取手术切除腺瘤伴不典型增生30例(中低度16例、重度14例)、腺癌50例、邻近正常黏膜50例,通过免疫组化、荧光原位杂交方法检测其Id-1的表达情况,并研究其与结直肠癌相关临床病理因素之间的关系。结果 Id-1蛋白在结直肠正常黏膜、中低度不典型增生腺瘤、重度不典型增生腺瘤、腺癌中的阳性表达率分别为24.00%、31.25%、57.14%、72.00%;Id-1mRNA在结直肠正常黏膜、中低度不典型增生腺瘤、重度不典型增生腺瘤、腺癌中的阳性表达率分别为20.00%、25.00%、57.14%、76.00%;Id-1蛋白及Id-1mRNA在正常黏膜组织、腺瘤伴中低度不典型增生、腺瘤伴重度不典型增生、腺癌中的阳性表达逐步增强(P<0.01);Kappa一致性检验显示,在正常黏膜、腺瘤、腺癌组织中吻合系数分别为k=0.791、0.729、0.905;从TNMⅠ~Ⅳ期Id-1表达也逐渐增强,差异有统计学意义(P<0.01);Id-1表达与肿瘤浆膜浸润、CEA(+)、淋巴结转移、肝转移及脉管浸润等密切相关(P<0.01),而与肿瘤大小及分化程度无关。结论 Id-1异常表达在肿瘤癌变过程中发挥了重要作用,有望成为新的肿瘤监测指标,为临床诊治及预后判断指明新的方向。
Objective To study the role of DNA-binding inhibitor of differentiation 1 (Id-1) in the proliferation and differentiation of colorectal cancer cells. Methods Thirty cases with atypical hyperplasia (16 cases in moderate and low grade, 14 cases in severe) were selected for surgical excision. Fifty cases of adenocarcinoma and 50 cases of adjacent normal mucosa were selected for immunohistochemistry and fluorescence in situ hybridization. Id-1 And to study the relationship between them and the clinicopathological factors related to colorectal cancer. Results The positive rates of Id-1 protein in colorectal mucosa, moderate and low-grade atypical hyperplasia adenocarcinoma, severe atypical hyperplasia adenocarcinoma and adenocarcinoma were 24.00%, 31.25%, 57.14% and 72.00%, respectively. Id- 1 mRNA in normal colorectal mucosa, moderate-low-grade atypical hyperplastic adenoma, severe atypical hyperplastic adenoma and adenocarcinoma were 20.00%, 25.00%, 57.14% and 76.00%, respectively. Id-1 protein and Id -1mRNA in normal mucosa, adenoma with low-grade atypical hyperplasia, adenoma with severe dysplasia, adenocarcinoma positive expression gradually increased (P <0.01); Kappa consistency test showed that in normal mucosa, gland The coincidence coefficients of Id-1 in tumor and adenocarcinoma were k = 0.791,0.729 and 0.905, respectively. Id-1 expression also gradually increased from TNMⅠtoⅣ (P <0.01) Infiltration, CEA (+), lymph node metastasis, liver metastasis and vascular invasion were closely related (P <0.01), but not with tumor size and degree of differentiation. Conclusion The abnormal expression of Id-1 plays an important role in the carcinogenesis of cancer and is expected to become a new indicator of tumor monitoring, indicating a new direction for clinical diagnosis and prognosis.