论文部分内容阅读
以还原糖产量和酶解效率为考查指标,对酶负荷、底物浓度、酶解时间等条件进行优化研究,并讨论纤维素酶重吸附再利用方法的可行性。结果表明:100mL反应液中,酶解预处理反应的最优工艺为酶负荷100mg、底物浓度25mg/mL、酶解时间48h,此条件下还原糖产量为1459mg,酶解效率为52.52%。利用酶解液进行光合微生物产氢实验,7d累积产氢量最高为1011mL。用底物吸附法进行纤维素酶重吸附利用,得出纤维素酶可进行3次高效再利用,对减少酶用量、降低制氢成本有重要意义。
The yield of reducing sugar and the efficiency of enzymatic hydrolysis were taken as indexes to study the conditions of enzyme loading, substrate concentration and enzymolysis time. The feasibility of cellulase reabsorption and reuse was discussed. The results showed that the optimum conditions of enzymatic pretreatment were enzyme loading 100mg, substrate concentration 25mg / mL and enzymolysis time 48h. The yield of reducing sugar was 1459mg and the enzymolysis efficiency was 52.52%. Hydrogen production experiments of photosynthetic microorganisms were carried out by enzymatic hydrolyzate. The maximum cumulative hydrogen production in 7 days was 1011 mL. The substrate adsorption method for cellulase reabsorption and utilization of cellulase obtained three times efficient reuse, reduce enzyme dosage, reduce the cost of hydrogen production is of great significance.