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目的观察中药安脑片对异体移植小鼠aGVHD的干预作用。方法 SPF级Balb/c雄性小鼠作为供鼠,以SPF级C57BL/6雌性小鼠为受鼠,建立aGVHD模型,分为安脑片组和空白组,每组10只。观察期间记录小鼠的一般状况及称质量、存活时间,给予临床aGVHD评价,移植后第30天杀鼠取材,制备肝脏、小肠、皮肤等标本,观察其组织学变化,以期评价其干预aGVHD的效果。结果空白组小鼠在移植后第15天全部死亡,死亡率100%,平均生存时间平均为11.9 d;安脑片组死亡3只,存活率70%,平均生存时间平均为27.3 d,差异显著(P=0.000)。安脑片组小鼠平均在移植后第12天精神状态开始恢复,喜动,饮食基本如常,体质量增加,毛发光泽,大便如常。安脑片组皮肤、腹泻、体质量减轻、姿势及活动度的临床评分分别为:0.10±0.32、0.20±0.42、0.12±0.00、0.10±0.10,总分为0.31±0.14,而空白组小鼠上述组织的临床评分分别为2.70±0.48、2.90±0.32、2.91±0.34、2.80±0.42,总评分为3.00±0.00,2组差异明显(P=0.026)。从组织病理学上,安脑片组小鼠肝脏、皮肤、小肠组织较空白组相比,均有不同程度的改善。结论安脑片可有效减轻异体移植小鼠aGVHD的症状和程度,延长小鼠存活时间,改善组织病理。
Objective To observe the intervention effect of An’nao Tablet on aGVHD in allograft mice. Methods SPF-grade Balb / c male mice were used as donors and SPF-C57BL / 6 female mice as recipients. The aGVHD model was established and divided into AnNao Tablet group and blank group, with 10 mice in each group. During the observation period, the general condition, quality and survival time of the mice were recorded, and the clinical aGVHD was evaluated. On the 30th day after transplantation, the mice were sacrificed and the liver, small intestine and skin specimens were prepared. The histological changes were observed to evaluate the effects of aGVHD effect. Results The mice in the blank group all died on the 15th day after transplantation, with a mean mortality of 100% and an average survival time of 11.9 days. Three mice died in the An Nao Tablet group with a survival rate of 70% and an average survival time of 27.3 days (P = 0.000). On the other hand, on the 12th day after transplantation, the mice in An Nao Tablet group began to recover their mental state on the 12th day after transplantation. The appetite was normal and the diet was basically normal. The body weight increased, the luster of hair and the stool were normal. The clinical scores of skin, diarrhea, weight loss, posture and activity of An Nao tablet group were 0.10 ± 0.32,0.20 ± 0.42,0.12 ± 0.00,0.10 ± 0.10 respectively, with a total score of 0.31 ± 0.14, while the blank group mice The clinical scores of the above tissues were 2.70 ± 0.48, 2.90 ± 0.32, 2.91 ± 0.34 and 2.80 ± 0.42 respectively, with a total score of 3.00 ± 0.00. There were significant differences between the two groups (P = 0.026). Histopathologically, the brain, liver, skin and intestinal tissue of mice in An Nao Tablet group showed different degrees of improvement compared with the blank group. Conclusions AnNao Tablet can effectively reduce the symptoms and degree of aGVHD in xenografted mice, prolong the survival time of mice and improve the histopathology.