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目的 观察减毒水痘病毒对乙型肝炎病毒复制的影响。方法分别用减毒水痘病毒接种鸭乙型肝炎模型和HepG2 2.2.15细胞，以斑点杂交和EIA方法分别检测鸭血清中DHBV DNA和细胞培养上清液中HBsAg、HBeAg的含量。结果减毒水痘病毒两个剂量组均显示鸭血清病毒量下降，200pfu/kg组在给药后第10天和停药5d时，DHBV DNA 吸光度（A）平均值分别由给药前1.17±0.29降至0.59±0.45和0.21±0.21，相比差异有显著性（t＝3.51, P＜0.01和t ＝7.54，P＜0.001）；400pfu/kg组在给药后第5、10天DHBV DNA无下降，停药5d时DHBV DNA A平均值由给药前0.70±0.25 降至 0.32±0.17，差异有显著性（t ＝3.58， P＜0.01）；减毒水痘病毒对2.2.15细胞分泌HBeAg、HBsAg均有抑制作用，最大抑制率分别为61%和33%，对HBeAg的抑制较HBsAg强。结论减毒水痘病毒在鸭乙型肝炎模型上可以显著降低血清DHBV DNA水平；在体外能够直接抑制2.2.15细胞分泌HBsAg 、HBeAg ，提示该病毒可能对乙型肝炎病毒复制有干扰或抑制作用。“,”Objective To observe the effect of attenuated varicella-zoster virus (VZV) on replication of HBV in vitro and vivo. Methods The attenuated VZV were inoculated into the ducks infected with DHBV and into the cultural HepG2 2.2.15 cells, respectively. DHBV DNA, HBsAg and HBeAg were detected from the duck serum and cell cultural fluid by dot-blot hybridization and EIA. Results The decline of DHBV DNA in duck serum was found in two dosage groups. Compared with pre-treatment, the level of AD value in 200 pfu/kg group was significantly decreased from 1.17±0.29 to 0.59±0.45 and 0.21±0.21 ( t =3.51, 7.54, P< 0.001). The level in 400 pfu/kg group was from 0.70±0.25 to 0.32±0.17( t =3.58, P<0.01 ), respectively after treatment. The inhibitory rates of HBeAg and HBsAg were 61% and 33%, respectively. The effect on HBeAg seems to be more obvious than on HBsAg. Conclusion Attenuated VZV could significantly decrease serum DHBV DNA in DHBV-infected ducks and directly inhibit the HBeAg and HBsAg secreted by 2.2.15 cells, suggesting that VZV seems to interfere or inhibit the replication of DHBV.