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目的探讨肉苁蓉提取物松果菊苷(ECH)延缓衰老的药理作用及其可能的作用机制。方法小鼠皮下注射10%D-GAL10 mL.kg-1,每天1次,连续8周,建立亚急性衰老模型。阳性组和ECH各组分别同时灌胃给予维生素E 40 mg·kg-1和ECH20,40,60 mg·kg-1,每天1次。检测小鼠抗应激能力、血清中IL-2、IL-6含量以及脑组织中一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果与正常组比较,模型组小鼠抗应激能力下降,血清IL-2含量和脑组织NO、SOD含量均显著下降,IL-6和脑组织MDA含量显著升高。ECH各剂量组和维生素E阴性对照组均能提高衰老小鼠抗应激能力、血清IL-2含量和脑组织NO、SOD含量,降低IL-6和脑组织MDA含量。结论 ECH能够提高衰老小鼠的免疫能力、NO含量,减轻D-GAL诱导衰老小鼠的活性氧自由基损伤,这些可能是其延缓衰老的机制之一。
Objective To investigate the pharmacological action and its possible mechanism of Cistanche extract extract echinacoside (ECH) against aging. Methods Mice were subcutaneously injected with 10% D-GAL 10 mL.kg-1 once daily for 8 weeks to establish a sub-acute aging model. The positive group and the ECH groups were orally administered with vitamin E 40 mg · kg-1 and ECH20, 40 and 60 mg · kg-1 orally simultaneously once daily. The anti-stress ability, IL-2 and IL-6 levels in serum and the content of nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA) Results Compared with the normal group, the anti-stress ability of the model group decreased, the content of IL-2 in serum and the content of NO and SOD in brain tissue decreased significantly, while the content of MDA in IL-6 and brain tissue increased significantly. Each dose of ECH and vitamin E negative control group can improve anti-stress ability, serum IL-2 level, NO and SOD in brain tissue and MDA content in IL-6 and brain tissue of aging mice. Conclusion ECH can improve immune capacity and NO content in aging mice and reduce the reactive oxygen species damage induced by D-GAL in aging mice, which may be one of the mechanisms of anti-aging.