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目前大鼠原位肝移植已被广泛应用于肝移植术后免疫学、缺血—再灌注损伤及脏器保存效果等研究。我们根据Kamada[1]及张文海[2]等报告的双套管法对肝移植模型的制作加以改进,效果较好。1 材料与方法1.1 材料 采用Wistar、SD两种远交系雄性大鼠(北京医科大学动物室提供,试验室
At present, orthotopic liver transplantation in rats has been widely used in immunology, ischemia-reperfusion injury and organ preservation effect after liver transplantation. According to the double cannula method reported by Kamada [1] and Zhang Wenhai [2], we improved the liver transplantation model and achieved better results. 1 Materials and Methods 1.1 Materials Wistar, SD two kinds of outbred male rats (Beijing Medical University animal room, laboratory