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采用自组装技术,以L-半胱氨酸为基底结合纳米金(Nano-Au),然后再固定抗体,利用L-半胱氨酸对多巴胺的直接电催化作用放大电信号,制备了无酶标记且无电子媒介体的h-IgG电流型免疫传感器.通过交流阻抗、差示脉冲技术考察了电极表面的电化学特性,并对该免疫传感器的作用机理及性能进行了详细研究.免疫反应发生后,将电极转入含多巴胺的缓冲溶液中,用差分脉冲伏安法(DPV)测得h-IgG线性范围为0.80~90 ng/mL,检出限为0.25 ng/mL.实验结果表明,该免疫传感器具有制备简单、灵敏度高、稳定性好、线性范围宽等优点.
Using self-assembly technique, Nano-Au was immobilized on L-cysteine substrate, then the antibody was immobilized and the signal was amplified by direct electrocatalysis of L-cysteine on dopamine. Labeled h-IgG current type immunosensor with no electron mediator.The electrochemical characteristics of the electrode surface were investigated by AC impedance and differential pulse techniques, and the mechanism and performance of the immunosensor were studied in detail. After the electrode was transferred to dopamine buffer solution, the linear range of h-IgG was 0.80-90 ng / mL and the detection limit was 0.25 ng / mL by differential pulse voltammetry (DPV). The experimental results showed that, The immunosensor has the advantages of simple preparation, high sensitivity, good stability and wide linear range.