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目的在恐惧心理应激实验中检测自制模拟地震法的实效性。方法实验设对照组、旁观电击组和模拟地震组,其中模型组分别采用自制模拟地震法及经典的旁观电击法制备恐伤母鼠动物模型,通过观察3组母鼠孕产情况及1月龄仔鼠认知记忆能力评判模型建立是否成功。结果与对照组比较,模拟地震及旁观电击组母鼠产仔数减少,差异均有统计学意义(P<0.05);与对照组比较,旁观电击组仔鼠4d平均逃避潜伏期[分别为(89.5±27.2)、(70.7±40.0)、(57.4±38.3)、(55.5±35.9)s]均延长,差异有统计学意义(P<0.05),与旁观电击组比较,模拟地震组仔鼠平均逃避潜伏期均缩短,差异有统计学意义(P<0.05);与对照组比较,旁观电击组仔鼠平均游泳速度变慢(P<0.05),在20%边缘区平均百分比增大(P<0.05),在其他区域平均百分比减小(P<0.05),差异均有统计学意义(P<0.05),模拟地震组仔鼠平均游泳速度减慢,差异有统计学意义(P<0.05);模拟地震组与旁观电击组比较,各指标差异均无统计学意义(P>0.05)。结论模拟地震法应用到恐惧心理应激实验中具有一定的可行性,但需进一步改进完善。
Objective To test the effectiveness of self-made simulated seismic method in fear psychological stress test. METHODS: The control group, bystander shock group and simulated earthquake group were set up. The animal models of the females were prepared by self-made simulated seismic method and classic bystander shock method respectively. Success or failure of cognitive assessment of pups. Results Compared with the control group, the number of litter size in the simulated earthquake and bystander shock group was significantly decreased (P <0.05). Compared with the control group, the average escape latency of the shock litter group 4d was [89.5 ± 27.2), (70.7 ± 40.0), (57.4 ± 38.3) and (55.5 ± 35.9) s, respectively, with statistical significance (P <0.05). Compared with the bystander shock group, (P <0.05). Compared with the control group, the average swim speed of the off-guard shock group was slower (P <0.05) and the average percentage of the marginal zone at 20% (P <0.05) (P <0.05), while the average percentage in other regions decreased (P <0.05), the difference was statistically significant (P <0.05). The mean swimming speed of pups in simulated earthquake group was slower than that in other regions There was no significant difference in each index between the group and the sideshock group (P> 0.05). Conclusion It is feasible to apply simulated seismic method to the experiment of fear psychological stress, but it needs further improvement and perfection.