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在pH2.9～4.6 Britton-Robinson(BR)缓冲溶液中,秋水仙碱的水解产物(H-COL)能与溴酚蓝(BPB)、溴甲酚绿(BCG)、溴百里酚蓝(BTB)和百里酚蓝(TB)等酸性磺酞类染料(ASPD)反应形成1∶1的离子缔合物,此时将引起共振瑞利散射(RRS)的急剧增强,并产生新的RRS光谱.秋水仙碱水解产物与溴酚蓝、溴甲酚绿、溴百里酚蓝和百里酚蓝形成离子缔合物的最大散射波长分别位于327,311,305和306nm处.散射增强(ΔI)与秋水仙碱浓度在一定范围内成正比,不同体系对于秋水仙碱的检出限(3σ)分别为12.3,15.1,16.4和20.0ng·mL-1(TB).研究了适宜的反应条件,考察了共存物质的影响,表明方法有较好的选择性.基于秋水仙碱水解产物与酸性磺酞类染料离子缔合物的反应,发展了一种较灵敏,且简便、快捷测定秋水仙碱的新方法.方法用于片剂、黄花、血清和尿样中秋水仙碱的测定,获得了满意的结果.
The colchicine hydrolyzate (H-COL) was able to react with bromophenol blue (BPB), bromocresol green (BCG), bromothymol blue BTB), and acid sulfophthalic dyes (ASPD) such as thymol blue (TBD) react to form a 1: 1 ion association that causes a sharp increase in Resonance Rayleigh scattering (RRS) and creates a new RRS Spectra The maximum scattering wavelengths for the formation of ionomers of colchicine hydrolysates with bromophenol blue, bromocresol green, bromothymol blue, and thymol blue are at 327, 311, 305 and 306 nm, respectively.Isolation enhancement (ΔI) The concentration of colchicine was proportional to a certain range, and the detection limits (3σ) were 12.3, 15.1, 16.4 and 20.0 ng · mL-1 (TB) for colchicine in different systems.The appropriate reaction conditions were investigated, Coexisting substances, indicating that the method has better selectivity.Based on the reaction of colchicine hydrolysates and acidic sulfonphthalein dye ion-association compounds, a more sensitive, simple and quick method for the determination of colchicine has been developed Methods The method was applied to the determination of colchicine in tablets, yellow, serum and urine samples with satisfactory results.