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Ａ蛋白基因载体质粒ｐＲＩＴ—５是一个Ｅ．ｃｏｌｉ和枯草杆菌（Ｂ．Ｓｕｂｔｉｌｉｓ）的穿梭质粒。它在大肠杆菌中呈现氨基苄青霉素抗药性（Ａｐｒ），氯霉素抗药性（Ｃｍｒ），在枯草杆菌中仅呈现Ｃｍｒ。利用细胞感受态转化及原生质体转化，将ｐＲＩＴ—５由大肠杆菌分别导入枯草杆菌Ｒ２５及地衣芽孢杆菌ＮＭ１０５，经Ｃｍｒ转化株筛选及酶切电泳，结果表明该Ａ蛋白基因载体质粒ｐＲＩＴ—５成功地转化至Ｒ２５及ＮＭ１０５中。根据Ａ蛋白基因产物—Ａ蛋白能特异地与许多动物的免疫球蛋白的Ｆｃ段结合这一特性，应用ＥＬＩＳＡ检测方法，测定Ａ蛋白基因在Ｒ２５及ＮＭ１０５中的表达情况。其结果表明，Ａ蛋白基因表达量与胞外蛋白酶活性关系极为密切。
A protein gene vector plasmid pRIT-5 is an E. shuttle plasmid for E. coli and B. subtilis. It exhibits ampicillin resistance (Apr), chloramphenicol resistance (Cmr) in E. coli and only Cmr in B. subtilis. The recombinant plasmid pRIT-5 was transfected into Bacillus subtilis R25 and Bacillus licheniformis NM105 respectively by cell competent transformation and protoplast transformation. The recombinant plasmid pRIT-5 was successfully transformed into plasmid pRIT-5 Transformed into R25 and NM105. According to the characteristic that A protein gene product-A protein can bind specifically to the Fc region of immunoglobulin of many animals, ELISA method was used to determine the expression of A protein gene in R25 and NM105. The results show that the protein A gene expression and extracellular protease activity is closely related.