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目的了解近年来无锡市不同来源的副溶血性弧菌的毒力基因携带情况、血清型和耐药性。方法对于分别来源于食源性疾病事件、哨点医院监测标本、外环境监测样品的92株副溶血性弧菌分离株,采用PCR进行毒力基因鉴定,玻片凝集试验进行血清分群,纸片法进行耐药试验。结果 92株副溶血性弧菌中,食源性疾病事件、哨点医院、外环境监测这三种来源的分离株中携带tdh基因的菌株分别占85.7%(12/14)、88.6%(39/44)和14.7%(5/34);食源性疾病事件分离株和哨点医院分离株血清群以O3为主,分别占比85.7%(12/14)和65.9%(29/44),外环境监测分离株无优势血清群;食源性疾病事件、哨点医院、外环境监测这三种来源的分离株中耐氨苄西林的菌株分别占35.7%(5/14)、65.9%(29/44)和11.8%(4/34)。不同来源的菌株对氯霉素、氨苄西林/舒巴坦、庆大霉素、亚胺硫霉素都表现为100.0%敏感。结论 2012—2014年无锡市从食源性疾病事件、哨点医院监测标本和外环境监测样品分离出的副溶血性弧菌在毒力基因、血清群、耐药情况上均有较大差异。建议结合耐药试验结果,在无锡市临床治疗副溶血性弧菌感染病例时,抗生素使用首选氯霉素、氨苄西林/舒巴坦、庆大霉素、亚胺硫霉素。
Objective To understand virulence genes, serotypes and drug resistance of Vibrio parahaemolyticus from different sources in Wuxi in recent years. Methods Ninety-two isolates of Vibrio parahaemolyticus from foodborne disease events, sentinel hospital surveillance samples and external environmental monitoring samples were used for virulence gene identification and slide agglutination test for serogroups, Method for drug resistance test. Results Among the 92 strains of Vibrio parahaemolyticus, 85.7% (12/14) and 88.6% (39.6%) of the isolates carrying tdh were found in foodborne disease events, sentinel hospitals and external environment monitoring, respectively / 44) and 14.7% (5/34) respectively. Serogroups of foodborne disease incidents isolates and sentinel hospital isolates were mainly O3, accounting for 85.7% (12/14) and 65.9% (29/44), respectively , Respectively. There was no dominant serogroup in the external environmental monitoring isolates; 35.7% (5/14) and 65.9% (5/14) of ampicillin-resistant isolates were found in the foodborne disease events, sentinel hospitals and external environment monitoring, respectively 29/44) and 11.8% (4/34). Strains from different sources showed 100.0% sensitivity to chloramphenicol, ampicillin / sulbactam, gentamicin, and thimidine. Conclusion Vibrio parahaemolyticus isolated from foodborne disease incidents, sentinel hospital surveillance specimens and external environmental monitoring samples from 2012 to 2014 in Wuxi showed significant differences in virulence genes, serogroups and drug resistance. Recommendations combined with drug resistance test results, the clinical treatment of Vibrio parahaemolyticus in Wuxi City, the case of antibiotics preferred chloramphenicol, ampicillin / sulbactam, gentamicin, imipenem.