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目的:观察灵芝多糖(GLPs)对2型糖尿病大鼠心肌组织中晚期糖基化终产物(advanced glycation end products,AGEs)及其受体(receptor for advanced glycation end products,RAGE)表达的影响,探讨其防治糖尿病大鼠心肌病变的作用机制。方法:采用高能量饮食加小剂量腹腔注射链脲佐菌素(STZ)的方法建立2型糖尿病大鼠模型(Type 2 Diabetes Mellitus,T2DM),成模后将大鼠随机分成6组:正常对照组、模型对照组、灵芝多糖低、中、高剂量治疗组、小檗碱对照组。灌胃治疗12周后测定各组大鼠空腹血糖、血浆胰岛素,免疫组化和蛋白印迹法检测心肌组织中AGEs、RAGE含量的表达。结果:与DM组比较,灵芝多糖组、小檗碱组能有效降低血糖、升高血浆胰岛素水平,降低心肌组织中AGEs、RAGE的表达,其中灵芝多糖高剂量组效果优于小檗碱组。结论:灵芝多糖可显著改善糖尿病大鼠血糖、空腹血浆胰岛素水平,并可能通过抑制心肌组织中AGEs、RAGE的表达实现其保护糖尿病大鼠心肌的作用。
Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GLPs) on the expression of advanced glycation end products (AGEs) and their receptors (RAGE) in myocardial tissue of type 2 diabetic rats The prevention and treatment of diabetic rats myocardial pathological mechanism. Methods: Type 2 Diabetes Mellitus (T2DM) was established by intraperitoneal injection of streptozotocin (STZ) with high-energy diet and low dose. Rats were randomly divided into 6 groups: normal control Group, model control group, Ganoderma lucidum polysaccharide low, medium and high dose treatment group, berberine control group. Fasting plasma glucose, plasma insulin, and the expression of AGEs and RAGE in myocardium were detected by immunohistochemistry and Western blotting after the rats were treated by gavage for 12 weeks. Results: Compared with DM group, Ganoderma lucidum polysaccharide group and berberine group could effectively decrease blood glucose, increase plasma insulin level and decrease AGEs and RAGE in myocardium. The effect of Ganoderma lucidum polysaccharide in high dose group was better than that in berberine group. CONCLUSION: Ganoderma lucidum polysaccharide can significantly improve blood glucose and fasting plasma insulin levels in diabetic rats and may protect myocardium of diabetic rats by inhibiting the expression of AGEs and RAGE in myocardium.