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目的 探讨中药刺五加 (AS)防治蛛网膜下腔出血 (SAH)后脑血管痉挛 (CVS)的作用机制。方法 采用犬枕大池二次注血制作实验性犬SAH后CVS动物模型。放免法测定SAH后 3 0min、2d、4d和 7d时血及脑脊液 (CSF)中内皮素 (ET)和降钙素基因相关肽 (CGRP)含量。并于第二次注血后 3 0min静脉滴注AS注射液 ,以后每日 1次 (40mL/d)至第 7天 ,测定时间点同上。结果 AS组在第二次注血后 2d、4d、7d血浆及CSF中ET含量较对照组高 ,较单纯注血组低。而CGRP较对照组略低 ,较注血组高。结论 刺五加可以通过抑制ET升高和CGRP降低而达到防治SAH后CVS的目的。
Objective To investigate the mechanism of action of Acanthopanax senticosus (AS) on cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH). Methods CVS animal model of experimental dogs after SAH was made by the second injection of dog’s pillow. The levels of endothelin (ET) and calcitonin gene related peptide (CGRP) in blood and cerebrospinal fluid (CSF) at 30 min, 2 d, 4 d and 7 d after SAH were determined by radioimmunoassay. AS injection was intravenously administered 30 minutes after the second injection of blood, and once daily (40mL / d) to day 7 after the injection. The time point was determined as above. Results The levels of ET in plasma and CSF were significantly higher in AS group than those in control group on the 2nd, 4th, and 7th day after the second injection of blood, which was lower than that of the simple injection group. The CGRP slightly lower than the control group, higher than the injection group. Conclusions Acanthopanax senticosus can achieve the purpose of preventing CVS after SAH by inhibiting the increase of ET and the decrease of CGRP.