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目的观察高压氧预适应(hyperbaric oxygen preconditioning,HBOP)对大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)后的神经保护作用并初步探讨其对骨桥蛋白(osteopontin,OPN)表达的影响。方法雄性SD大鼠72只,分为假手术组,HBOP+假手术组,单纯MCAO组、HBOP+MCAO组,每组18只。采用大脑中动脉线栓法建立大鼠MCAO模型。于术后12 h取材,观察大鼠神经功能和脑梗死变化情况;HE染色光镜下观察缺血区脑组织的病理形态学改变。Western blot检测缺血区OPN的表达变化。结果神经功能缺损评分:术后12 h假手术组及HBOP+假手术组评分均为0分,单纯MCAO组(3.80±0.79)分,HBOP+MCAO组(2.50±0.97)分;平衡能力评分:术后12 h假手术组及HBOP+假手术组大鼠平衡能力评分均为5分,单纯MCAO组(1.50±1.08)分,HBOP+MCAO组(2.70±2.95)分;脑梗死率:术后12 h假手术组及HBOP+假手术组大鼠梗死率均为0,单纯MCAO组(49.22±7.07)%,HBOP+MCAO组(41.96±3.15)%。可见与单纯MCAO组比较,HBOP+MCAO组在术后12 h神经功能缺损评分明显降低(P<0.05),平衡能力评分显著提高(P<0.05);脑梗死率明显小于单纯MCAO组(P<0.05);HE染色见缺血区脑组织病理损伤明显减轻;Western blot检测提示OPN的表达显著增强。结论高压氧预适应可以诱导大鼠MCAO后的神经保护作用,其机制可能与HBOP引起的OPN表达增高有关。
Objective To observe the neuroprotective effect of HBOP on middle cerebral artery occlusion (MCAO) in rats and to explore its effect on osteopontin (OPN) expression. Methods Seventy two male Sprague Dawley rats were randomly divided into sham operation group, HBOP + sham operation group, MCAO group and HBOP + MCAO group, with 18 rats in each group. The rat MCAO model was established by middle cerebral artery occlusion. The rats were sacrificed at 12 h after operation to observe the changes of neurological function and cerebral infarction in rats. The pathological changes of brain tissue were observed under light microscope. Western blot was used to detect the expression of OPN in ischemic area. Results The score of neurological deficit score was 0 at sham operation group and HBOP + sham operation group at 12 h after operation. The score of MCAO group (3.80 ± 0.79) and HBOP + MCAO group (2.50 ± 0.97) After 12 h of sham operation and HBOP + sham operation group, the balance ability score was 5 points in the MCAO group (1.50 ± 1.08) and HBOP + MCAO group (2.70 ± 2.95); The cerebral infarction rate was 12 h The infarction rate in sham operation group and HBOP + sham operation group were all 0, 49.22 ± 7.07% in MCAO group and 41.96 ± 3.15% in HBOP + MCAO group. Compared with MCAO group, the score of neurological deficit in HBOP + MCAO group was significantly lower at 12 h after operation (P <0.05), and the balance ability score was significantly higher (P <0.05). The infarction rate in HBOP + MCAO group was significantly lower than that in MCAO group (P < 0.05). HE staining showed that the ischemic brain tissue pathological injury was significantly reduced; Western blot showed that OPN expression was significantly enhanced. CONCLUSION: Hyperbaric oxygen preconditioning can induce neuroprotective effect after MCAO in rats. The mechanism may be related to the increase of OPN expression induced by HBOP.